Journal: Animal nutrition (Zhongguo xu mu shou yi xue hui)
Article Title: Dietary lycopene supplementation improves meat quality, antioxidant capacity and skeletal muscle fiber type transformation in finishing pigs.
doi: 10.1016/j.aninu.2021.06.012
Figure Lengend Snippet: Fig. 3. Effect of dietary lycopene supplementation on AMP-activated protein kinase (AMPK) signaling pathway in longissimus dorsi (LD) muscle of finishing pigs. (A) The protein expressions of AMP-activated protein kinase (AMPK) and phospho-AMP-activated protein kinase (p-AMPK). (B) The protein expressions of nuclear respiratory factor 1 (NRF1), calcium/calmodulin-dependent protein kinase b (CaMKKb), sirtuin1 (Sirt1) and peroxisome proliferator activated receptor-g coactivator-1a (PGC-1a). (C) The mRNA expressions of AMP-activated protein kinase a1 (AMPKa1), AMP-activated protein kinase a2 (AMPKa2), sirtuin1 (Sirt1) and peroxisome proliferator activated receptor-g coactivator-1a (PGC-1a). Data are presented as the mean and SEM (n ¼ 6). a, b, c Bar with different letter indicates significant difference (P < 0.05).
Article Snippet: Membranes were blocked with 5% BSA in 1 TBST (20 mmol/L TriseHCl pH 7.6, 8 g/L NaCl, and 0.1% Tween-20) and then incubated with primary antibodies at 4 C for 16 h. The primary antibodies used were as follows: slow MyHC (1:500, Sigma, Cat. No. M8421), fast MyHC (1:500, Sigma, Cat. No. M4276), Myoglobin (1:1,000, Cell Signaling, Cat. No. 25919), cytochrome c (Cytc, 1:1,000, ProteinTech Biotechnology, Cat. No. 10993-1-AP), nuclear respiratory factor 1 (NRF1, 1:1,000, Cell Signaling, Cat. No. 46743), calcium/calmodulindependent protein kinase b (CaMKKb, 1:1,000, Cell Signaling, Cat. No. 16810), phospho-AMPK(p-AMPK, 1:1,000, Cell Signaling, Cat. No. 2535), AMPK (1:1,000, Cell Signaling, Cat. No. 5831), sirtuin1 (Sirt1, 1:1,000, Cell Signaling, Cat. No. 8469), peroxisome proliferator activated receptor-g coactivator-1a (PGC-1a, 1:800, Cell Signaling, Cat. No. 2178) and b-actin (1:1,000, Cell Signaling, Cat. No. 4967) antibodies.
Techniques: